Frequency of regulatory T cells in the spleen and CNS of mice with EAE: effect of previous infections with S. aureus. The percentage of CD4 + CD25 + Foxp3+ T cells was evaluated in the spleen (a) and CNS (b). Brain inflammatory infiltrates in mice previously infected with S. aureus TSST-1 (c) or with S. aureus TOX- strain (d) were evaluated 15 days after the infection. Data were expressed as the mean ± SE of 6-9 mice in Figures 7a and b. Different letters represent significant differences between groups. The statistical analysis (ANOVA with Dunn’s or Holm-Sidak method) indicates significant differences between EAE and both infected groups, p<0.05. Micrographs (c and d) are representative of 5-6 animals/group.
França et al. BMC Neuroscience 2014 15:8 doi:10.1186/1471-2202-15-8