Quantitation of necrotic cells under hydrostatic pressure and Nec-1 usage. Panel A: Normal control cells; Panel B: RGC-5 cell lines necrosis under elevate hydrostatic pressure; Panel C: RGC-5 cell lines were treated with necrostatin-1 (10 μmol/L) to block necroptosis for 24 hr in cell density of 0.6 × 105/ml before elevate hydrostatic pressure and analysis of necroptotic cells. Cells were stained with Annexin FITC and PI, and analyzed by FACS using FL1 (Annexin) and FL3 (PI) channels. Panel D: The statistical analysis of RGC-5 necrosis, * vs # p < 0.05.
Shang et al. BMC Neuroscience 2014 15:63 doi:10.1186/1471-2202-15-63