Additional file 3.


Ex vivo time-lapse of cGFP-expressing cells typical of astrocytes revealed their dynamic behaviour. cGFP expressing neurospheres were transplanted into the cord 12 days previously. The non-fixed cord was removed and visualised as ex vivo tissue by time lapse over a short time frame. A cGFP tagged astrocyte-like cell could be seen to change the pattern of GFP localisation which suggested a dynamic spontaneous extension of a cell process.

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Ioannidou et al. BMC Neuroscience 2014 15:59   doi:10.1186/1471-2202-15-59