T2-weighted images (T2WIs), diffusion-weighted images (DWIs), and ADC maps after PT and analysis of brain damage. (A) Serial T2WIs, DWIs and ADC maps of a representative mouse at 5 h, 1, 3, 6, 10, and 14 days after ischemia. In T2WIs (A1), arrowheads indicate the hyperintense signal in penumbra, star isointense signal in ischemic core, and dashed line arrow indicates glial scar formation. The boxed region in T2WI on day 3 was a hemorrhagic transformation. The midlines are indicated by dashed lines in T2WIs. In DWIs and ADC maps (A3-A4), arrowheads indicate vasogenic and cytotoxic edema. (B-C) Summary of brain infarct volumes based on T2WIs and DWIs. (D) The brain edema progression calculated as the ratio of ADC values in the ischemic region to the ADC values in the mirror region in contralateral hemisphere. (E) Time course of the ratio of ipsilateral to contralateral hemisphere volumes (IH/CH) based on T2WIs. All the data were averaged values from 4 mice. *p<0.05, ANOVA test. In B-C, comparisons are indicated in the figures, and in D-E, values were compared with control.
Li et al. BMC Neuroscience 2014 15:58 doi:10.1186/1471-2202-15-58