Smoke Preparation according to ISO/FTC protocol. Concentrated Smoke solution was prepared from each cigarette using CSM-SCSM Cigarette Smoking Machine according to ISO/FTC determination parameters. These require a puff volume of 35 ml with duration of 2 s at interval of 60s.
Naik et al. BMC Neuroscience 2014 15:51 doi:10.1186/1471-2202-15-51