Hydrogen Peroxide content profiling of CSE from tested tobacco products. (A1) H2O2 content increased proportionally to the amount of tar in the cigarettes where statistically significant higher H2O2 was found in full flavor, ultralow nicotine and nicotine free cigarettes than light cigarette (P < 0.001), (n = 10 CSE preparations). (A2) Immunofluorescence analysis of oxidative stress in ECs (HCMEC/D3 cell line) caused by CSE exposure from 1R5F, 3R4F, ultralow nicotine and NF cigarettes versus controls: Note that most significant oxidative responses were observed in EC cultures exposed to CSE treatment (24 h) derived from 3R4F, ultralow nicotine and NF cigarette (n = 3 biological replicates). Regression analysis of H2O2 correlates with tar (B) but not nicotine content unless ultralow nicotine and NF cigarettes are removed from the analytical pool (C).
Naik et al. BMC Neuroscience 2014 15:51 doi:10.1186/1471-2202-15-51