Figure 1.

HPLC and viability studies to select the CSE concentration for the study. A) HPLC analysis to determine nicotine concentration in CSEshowed that 5% CSE had nicotine concentration comparable to the physiological concentration in a chronic smoker (100 ng/ml) nā€‰=ā€‰10. (B) Cell viability studies following increasing concentration of nicotine at 24 and 48 h and (C) 5% diluted CSE from ultralow, 1R5F (equivalent to ultralight cigarettes), 3R4F (equivalent to full flavor cigarettes), ultralow nicotine and tobacco free (nicotine free - NF) using MTT assay. Note that 5% CSE from all tested brand but NF did not cause a statistically significant decrease in cell viability. nā€‰=ā€‰3 individual experiments.

Naik et al. BMC Neuroscience 2014 15:51   doi:10.1186/1471-2202-15-51
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