Chandler/RML neuropathology. Histological features of Chandler/RML prion transmission to Cx3cr1-/-(A-E) and wild type control (F-J) mice. Panels A and F show the global distribution of disease-associated PrP using anti-PrP monoclonal antibody ICSM35. Panels B, C, G and H show staining of microglia with Iba-1 in the hippocampus (B, G) and the thalamus (C, H). Panels D, E, I and J are stained with haematoxylin and eosin (H&E) to visualise spongiosis and neuronal loss in the hippocampus. Boxes show where the higher power images have been taken and arrows highlight apoptotic cells. Overall, no differences are seen between Cx3cr1-/- and wild type mice. Scale bar corresponds to 2 mm (A, F), 400 μm (B, C, D, G, H, I) or 100 μm (E, J).
Grizenkova et al. BMC Neuroscience 2014 15:44 doi:10.1186/1471-2202-15-44