Representative coronal images containing the BLA (~ Bregma -3.3) depicting AAV-RFP viral transduction for selected AAV serotypes (RFP fluorescence) and NeuN immunohistochemistry (FITC fluorescence). In this experiment, AAV-RFP virus was pseudotyped as AAV2/1, 2/5, 2/7, 2/8, 2/9, 2/rh10, 2/DJ, or 2/DJ8 and infused into the BLA at the titer of 7.8E + 11 GC/ml. Images of coronal brain sections containing the BLA are arranged for each serotype as follows: AAV-RFP image (scale bar = 100 μm), NeuN-FITC image and an image of these two previous images merged. Arrows point to a subset of NeuN positive cells that are also AAV-RFP positive.
Holehonnur et al. BMC Neuroscience 2014 15:28 doi:10.1186/1471-2202-15-28