Localization of mitochondrial EYFP and APP in C2C12 myotubes. (A) Cells were co-transfected with DNA constructs (described in Material and Methods) then incubated with mitotracker (red, left panel) or APP antibody (red, right panel) and dapi (blue). The enhanced yellow fluorescent protein (EYFP) is in green and co-localizes with mitotracker (left panel) but not APP (right panel). (Magnification 40x). (B) Representative OCR (pMoles/min) for transfected C2C12 myotubes (left panel). Solid black line (Control cells) and dotted line (Transfected cells). Average OCR (pMoles/min) for 3 separate cultures (right panel). Black bars (Control cells) and white bars (Transfected cells). Data are presented as the average OCR ± SE. Arrows represent successive additions of oligomycin (1 μg/ml), FCCP + pyruvate (400 nM + 200 μM respectively), and antimycin A (1 μM).
Schuh et al. BMC Neuroscience 2014 15:24 doi:10.1186/1471-2202-15-24