Full-length, transgene-derived amyloid precursor protein (APP) levels in brain homogenates. (A) Representative Western blots of homogenates isolated from the brains of App(swe)/PS1(ΔE9) (DTG) and non-transgenic (NTG) male mice (3 and 6 mo) probed with 6E10 antibody. (B) Transgene-derived, full-length APP (~106 Kd) is observed in DTG mice at both ages tested with negligible levels in the NTG mice. Full-length APP levels (ratio of APP:β-Actin) are significantly elevated in the 6 mo DTG mice (white bar) compared to NTG (black bar). Data are presented as the average full-length APP ± SE. N = 3 separate animals per genotype. *p < 0.01.
Schuh et al. BMC Neuroscience 2014 15:24 doi:10.1186/1471-2202-15-24