ROS-mediated activation of Drosophila larval nociceptor neurons by UVC irradiation
1 Department of Molecular Physiology and Biophysics, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USA
2 Current address: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
BMC Neuroscience 2014, 15:14 doi:10.1186/1471-2202-15-14Published: 16 January 2014
Larval writhing behavior in response to UVC(H) irradiation. Video showing an individual ppk1GAL4/+ control larva on an agarose plate exposed to UVC(H) irradiation at the indicated timepoint. Larva shows strong writhing behavior instaneous to UVC(H) exposure onset. Subsequent frames show an individual ppk1GAL4/UAS-TNT-G larva exposed to the same dose of UVC(H) irradiation at the indicated timepoint. Transgenic inactivation of mdIV neurons in the ppk1GAL4/UAS-TNT-G larva causes a complete suppression of the writhing response.
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Larval writhing behavior in response to noxious heat. Video showing an individual ppk1GAL4/+ control larva touched at midbody length with a probe heated to 42°C. The wild-type control larva shows a strong writhing behavior response as previously reported . Subsequent frames show an individual ppk1GAL4/UAS-TNT-G larva exposed to the same noxious heat probe at the indicated timepoint. Transgenic inactivation of mdIV neurons in the ppk1GAL4/UAS-TNT-G larva causes a complete suppression of the writhing response. Comparison of noxious heat induced writhing with UVC(H) induced writhing (Additional file 1) shows that the two responses are very similar if not identical.
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Additional file 3: Figure S1:
Expression patterns of tissue-specific GAL4 driver transposons. All stocks carrying tissue-specific GAL4 driver transposons were crossed with UAS-mCD8GFP to visualize and evaluate expression patterns. Indicated tissues were dissected from GAL4/UAS-CD8GFP larvae and imaged using an LSM710 confocal microscope.
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Additional file 4: Figure S2:
Effects of catalase overexpression on mdIV dendritic morphology. mdIV dendritic morphology in (A)ppk1GAL4/UAS-mCD8GFP or (B) ppk1GAL4/UAS-mCD8GFP; UAS-Cat/+ larvae. Confocal images of GFP fluorescence were obtained from living larvae to represent the overall dendritic arbor of two adjacent mdIV neurons. No gross defects in dendritic morphology are detected after catalase overexpression in mdIV neurons.
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