Methods. Illustration of the ventral aspect of the rat facial soft tissues (A) and a magnified view of the lower lip and skin innervation territory of the mental nerve (B). A partial injury of the left mental nerve was performed either by loosely ligating it with two 6/0 chromic gut sutures or by tightly ligating ⅓ to ½ of its diameter with a single 6/0 silk suture. The areas stimulated for behavioural testing are outlined and the tissue harvested for NGF quantification and innervation density measurement are also indicated.
Evans et al. BMC Neuroscience 2014 15:1 doi:10.1186/1471-2202-15-1