Molecular characterization of HD transgenic monkey founders. A) Schematic of lentiviral transgene for the generation of HD monkeys. Primers used to genotype the HD monkeys are depicted in the schematics. B) Genotyping of HD monkeys using the primers outlined in panel A. Expected PCR amplicon sizes are detailed in the table. Genotyping was performed using DNA extracted from blood samples of individual founder and control monkeys. C) A table describing the promoter, transgene structure, copy number and number of polyQ repeat units integrated into the genome of each HD monkey. All transgene copy number and polyQ analysis was examined in blood samples collected from each monkey. D) Fold change of HTT mRNA expression by qPCR in all HD monkeys compared to age-matched controls from blood. The qPCR for fold change analysis was set up in technical duplicates for each sample from the individual monkeys. The control group consists of the values for all 4 wild-type monkeys.
Kocerha et al. BMC Neuroscience 2013 14:88 doi:10.1186/1471-2202-14-88