Open Access Highly Accessed Research article

Longitudinal transcriptomic dysregulation in the peripheral blood of transgenic Huntington’s disease monkeys

Jannet Kocerha124, Yuhong Liu1, David Willoughby3, Kumaravel Chidamparam3, Joseph Benito3, Kate Nelson1, Yan Xu12, Tim Chi1, Heidi Engelhardt1, Sean Moran1, Shang-Hsun Yang1, Shi-Hua Li2, Xiao-Jiang Li2, Katherine Larkin1, Adam Neumann1, Heather Banta1, Jin Jing Yang1 and Anthony WS Chan12*

Author Affiliations

1 Department of Neuropharmacology and Neurologic Disease, Yerkes National Primate Research Center, 954 Gatewood Rd., N.E, Atlanta, GA 30329, USA

2 Department of Human Genetics, Emory University School of Medicine, 615 Michael St. Whitehead Building, Atlanta, GA 30322, USA

3 Ocean Ridge Biosciences, 10475 Riverside Drive, Suite 1, Palm Beach Gardens, FL 33410, USA

4 Current address: Department of Chemistry, Georgia Southern University, Statesboro, GA 30458, USA

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BMC Neuroscience 2013, 14:88  doi:10.1186/1471-2202-14-88

Published: 17 August 2013

Additional files

Additional file 1:

Wild-type rhesus macaque polyQ sequence. The polyQ sequence from the UCSC genome database was further confirmed and aligned with polyQ sequence from a wild-type rhesus housed at the Yerkes National Primate Center.

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Additional file 2:

HD rating scores. HD rating scores by HDPMRS at between 12 and 37 months for all HD and control monkeys.

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Additional file 3:

Microarray data. Raw fluorescence values for each sample at each timepoint from the Affymetrix Rhesus Macaque array analysis.

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Additional file 4:

Ingenuity analysis of HD monkey profiling results. The Ingenuity pathway program was used to identify signaling pathways (P value < 0.05) associated with HD mRNA transcripts dysregulated with a P value < 0.001 in the Affymetrix profiling.

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Additional file 5:

Human HD candidate graphs. Eleven selected transcripts from human HD blood array data (obtained from the GEO database) were graphed from control, pre-symptomatic, and post-symptomatic patient samples.

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Additional file 6:

Human HD array probeset information. The human array probeset information for the selected HD-associated candidates.

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Additional file 7:

qPCR expression analysis of selected HD mRNA candidates at extended timepoints. The mRNA expression of eleven candidates with HD association in monkey and human blood were analyzed at additional timepoints of 29, 32, and 39 months.

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Additional file 8:

HD Rating Scale. Parameters measured in the HD primate rating scale.

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Additional file 9:

Rhesus Taqman assay information. All forward, reverse, and probe sequences for rhesus Taqman assays engaged for this manuscript.

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Additional file 10:

Microarray methods. Additional information regarding the Rhesus Macaque Affymetrix profiling are outlined.

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