Indomethacin restores LTM failure in laminarin treated animals. A. Protocol indicating timing of injection and start of pre- and post training tests with respect to the start of the first training session for both the conditioned (CS-UCS) and control (CS-DS) animals. B. LTM assessment in animals treated with either vehicle, laminarin, indomethacin, a combined treatment of laminarin plus indomethacin (lamin + indo) or a combined treatment of laminarin plus NDGA (lamin + NDGA). None of the laminarin treated conditioned animals and laminarin + NDGA conditioned animals or any of the unconditioned animals responded with significant feeding movements upon amyl acetate application in the post-training test. Indomethacin only treated animals showed a significant increase in their Δrasp values compared to their unconditioned peers but this conditioned response was significantly smaller than observed in the vehicle conditioned animals. Both vehicle and laminarin + indomethacin treated conditioned animals responded with a significant increase in the Δrasp values compared to their unconditioned peers and the laminarin conditioned animals. This suggests that co-treatment of laminarin with a COX inhibitor reverses the laminarin induced adverse effect on LTM performance. ** = p < 0.01, *** p < 0.001, ns = not significant.
Hermann et al. BMC Neuroscience 2013 14:83 doi:10.1186/1471-2202-14-83