Table 2

The CUS procedure
Time Course of the experiment
The 1st day 9:00 Cage rocking (5 times per second) for 15 min
The 2nd day 9:00 Swimming in cold (4°C) water for 5 min
The 3rd day 9:00 Soiled cage (250 ml of tap water into the sawdust bedding) for 24 h
The 4th day 9:00 Lights on overnight
The 5th day 9:00 Cage tilting for 24 h
The 6th day 9:00 Restraint for 12 h
The 7th day 8:00 Food and water deprivation for 24 h
The 8th day 8:00 One-bottled-sucrose test sessions (last 1 h)
The 8th day 9:00 Soiled cage for 24 h
The 9th day 9:00 Electric stimulus (1.0 mA, each time for 1 second, 10 times per minute) for 5 min
The 10th day 9:00 Elevated temperature (40°C) for 15 min
The 11th day 9:00 Cage rocking for 15 min
The 12th day 9:00 Lights on overnight
The 13th day 9:00 Restraint for 12 h
The 14th day 8:00 Food and water deprivation for 24 h
The 15th day 8:00 One-bottled-sucrose test sessions (last 1 h)
The 15th day 9:00 Lights on overnight
The 16th day 9:00 Swimming in cold water for 5 min
The 17th day 9:00 Restraint for 12 h
The 18th day 9:00 Electric stimulus for 5 min
The 19th day 9:00 Cage rocking for 15 min
The 20th day 9:00 Soiled cage for 24 h
The 21th day 9:00 Cage tilting for 24 h (The CUS procedure finish)
The 22th day 6:30 Weighed body weight and open field test
The 22th day 8:00 Food and water deprivation for 24 h
The 23th day 8:00 One-bottled-sucrose test sessions (last 1 h)

Huang et al.

Huang et al. BMC Neuroscience 2013 14:67   doi:10.1186/1471-2202-14-67

Open Data