Figure 3.

Isolating AMPA-receptors contribution. A: Superimposed suprathreshold corticostriatal responses in a dSPN in control (red trace, control) and after adding 25 μM GYKI 52466 (GYKI) to the superfusion (black trace, +GYKI) to block AMPA-receptors while affecting, as less as possible, KA-receptors. B: Superimposed suprathreshold responses to intrastriatal stimulation in a dSPN in control (red trace) and after adding GYKI to the superfusion (black trace). C: Histogram summarizing results from a sample of neurons: the GYKI-sensitive fraction of corticostriatal response in dSPNs represents a 18% reduction (**P < 0.01), while GYKI-sensitive fraction of intrastriatal response in dSPNs represents a 55% reduction (***P < 0.001). D: Digital subtraction of the GYKI-sensitive component from corticostriatal response shows both an early and a late response, indicating contributions to both mono- and poly-synaptic responses in dSPNs. E: Digital subtraction of GYKI-sensitive component from response to intrastriatal stimulation mainly shows the initial response (previously reported AMPA-mediated PSP). F: Superimposed suprathreshold corticostriatal responses in an iSPN in control (green trace) and after adding GYKI to the superfusion (black trace). G: Superimposed suprathreshold responses to intrastriatal stimulation in an iSPN in control (green trace) and after adding GYKI to the superfusion (black trace). H: Histogram representing results from a sample of similar experiments: GYKI-blockade represented about 21% reduction (**P < 0.01) for corticostriatal responses in iSPNs, while it represented about 36% reduction of responses to intrastriatal stimulation (**P < 0.01). I: Digital subtraction of GYKI-sensitive component from corticostriatal response in iSPNs shows early and late components. J: Digital subtraction of GYKI-sensitive component from intrastriatal response in iSPNs mainly shows the previously reported AMPA-mediated PSP.

Vizcarra-Chacón et al. BMC Neuroscience 2013 14:60   doi:10.1186/1471-2202-14-60
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