Contribution of AMPA/KA-receptors in corticostriatal suprathreshold responses of SPNs. A: Photomicrographs of a double labeled recorded neuron. Superimposition of eGFP-green and injected biocytin-red-CY3 for a D1- eGFP. B: Similar photomicrograph for a D2-eGFP neuron (scales = 10 μm). C: Superimposed suprathreshold corticostriatal responses in a dSPN in control (red trace) and after adding 10 μM CNQX to the superfusion (black trace). D: Superimposed suprathreshold corticostriatal responses in an iSPN in control (green trace) and after adding CNQX to the superfusion (black trace). The voltage-clamp recording in the inset shows that a cortical stimulus is followed by an initial PSC and a late barrage of PSCs that may last hundreds of milliseconds (stimulus strength was tuned up to reveal synaptic components only). E: Histogram representing a sample of neurons shows that the CNQX-sensitive fraction of corticostriatal response in both dSPNs and iSPNs is about 40% in each case (***P < 0.001; *P < 0.05). F: Digital subtraction of the CNQX-sensitive fraction from corticostriatal response in dSPNs shows two components (arrows): an early fast rising PSP followed by a late slow depolarization, suggesting polysynaptic arrival. G: Digital subtraction of CNQX-sensitive fraction from corticostriatal response in iSPNs shows two components (arrows): an initial fast PSP followed by a late slow plateau, which underlies the corticostriatal response.
Vizcarra-Chacón et al. BMC Neuroscience 2013 14:60 doi:10.1186/1471-2202-14-60