Additional file 1.

Negative controls for specificity of primary and secondary antibodies. Specificity of (A) the monoclonal rat anti-human α-Syn antibody and (B) the pan-specific monoclonal mouse anti-human and anti-murine α-Syn antibody were tested on frontal sections through the neocortex (Cx), corpus callosum (cc) and caudate/putamen (CPu) of α-Syn knockout mice. We also tested specificity of secondary antibodies (C-E) on sections through the hippocampal formation of D-Line mice; the panels show residual fluorescence in the CA3 region after omitting primary antibodies. Only low level background fluorescent signal was detected in all control experiments. Abbreviations: stratum oriens (so), stratum pyramidale (sp), stratum radiatum (sr). Scale bar = 70 μm (A, B), 50 μm (C-E).

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Amschl et al. BMC Neuroscience 2013 14:6   doi:10.1186/1471-2202-14-6