Open Access Highly Accessed Research article

Time course and progression of wild type α-Synuclein accumulation in a transgenic mouse model

David Amschl1, Jörg Neddens1, Daniel Havas1, Stefanie Flunkert1, Roland Rabl1, Heinrich Römer2, Edward Rockenstein3, Eliezer Masliah3, Manfred Windisch1 and Birgit Hutter-Paier1*

Author Affiliations

1 QPS Austria GmbH, Parkring 12, Grambach, 8074, Austria

2 Karl Franzens University, Institute of Zoology, Graz, 8010, Austria

3 Department of Pathology, University of California San Diego, La Jolla, CA, USA

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BMC Neuroscience 2013, 14:6  doi:10.1186/1471-2202-14-6

Published: 9 January 2013

Additional files

Additional file 1:

Negative controls for specificity of primary and secondary antibodies. Specificity of (A) the monoclonal rat anti-human α-Syn antibody and (B) the pan-specific monoclonal mouse anti-human and anti-murine α-Syn antibody were tested on frontal sections through the neocortex (Cx), corpus callosum (cc) and caudate/putamen (CPu) of α-Syn knockout mice. We also tested specificity of secondary antibodies (C-E) on sections through the hippocampal formation of D-Line mice; the panels show residual fluorescence in the CA3 region after omitting primary antibodies. Only low level background fluorescent signal was detected in all control experiments. Abbreviations: stratum oriens (so), stratum pyramidale (sp), stratum radiatum (sr). Scale bar = 70 μm (A, B), 50 μm (C-E).

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