Barrier function demonstrated by RSCEC monolayers co-cultured on cell culture inserts with mixed glial cells. Relationship between pre-experimental TEER, measured at room temperature, and permeability to Lucifer yellow over 90 minutes at 37°C in the optimised EBM-2/EGM-2 media conditions. Data was fitted to a one-phase exponential decay curve, R2 = 0.91, n = 8 independent cell culture experiments, 24 cell culture inserts in total.
Watson et al. BMC Neuroscience 2013 14:59 doi:10.1186/1471-2202-14-59