Table 2

Mating, copulation duration and fertility phenotypes of FRUM-depleted males
Male genotype Percent mated (%) Mean copulation duration ± SEM Percent fertile (%)
WT 67 (n = 19) 15.6 ±1.0 (n = 19) 88 (n = 16)
En-GAL4/+ 85 (n = 20) 15.3 ± 0.53 (n = 20) 84 (n = 16)
fruMRNAi/+, UAS-GAL4-I /+ 95 (n = 20) 16.7 ± 1.0 (n = 20) 90 (n = 20)
En-fruMRNAi 57 (n = 28) 10.9 ± 1.5 (n = 13) 61 (n = 23)
En-fruMRNAi, UAS-GAL4-I 19 (n = 21) 11.3 ± 4.4 (n = 21) 25 (n = 20)
En-fruMRNAi, UAS-GAL4-II 35 (n = 20) 12.9 ± 2.2 (n = 20) 40 (n = 20)
En-fruMRNAi, UAS-GAL4-III 55 (n = 20) 13.0 ±1.6 (n = 20) 67 (n = 21)
En-fruMRNAi, Cha-GAL80 30 (n = 20) 19.0 ± 3.9 (n = 20) 42 (n = 19)

All males were raised and maintained at 29°C. Single males (n = number tested) and females were paired for 30 minutes and the percent mated and the mean copulation duration calculated in minutes. Fertility tests were carried out at 29°C between an individual male and 2–3 virgin WT females for 7 days and the presence of larvae determined. For these experiments, the En-GAL4 chromosome had been backcrossed into a w- background for four generations.

Latham et al.

Latham et al. BMC Neuroscience 2013 14:57   doi:10.1186/1471-2202-14-57

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