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Open Access Highly Accessed Research article

A small cohort of FRUM and Engrailed-expressing neurons mediate successful copulation in Drosophila melanogaster

Kristin L Latham123*, Ying-Show Liu14 and Barbara J Taylor1

Author Affiliations

1 Department of Zoology, Molecular and Cellular Biology Program, Oregon State University, Corvallis, Oregon 97331-2914, USA

2 Molecular and Cellular Biology Program, Oregon State University, Corvallis, Oregon 97361, USA

3 Present address: Biology Department, Western Oregon University, Monmouth, Oregon 97361, USA

4 Present address: National Health Research Institutes, Zhunan, Miaoli 35053, Taiwan

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BMC Neuroscience 2013, 14:57  doi:10.1186/1471-2202-14-57

Published: 21 May 2013

Abstract

Background

In Drosophila, male flies require the expression of the male-specific Fruitless protein (FRUM) within the developing pupal and adult nervous system in order to produce male courtship and copulation behaviors. Recent evidence has shown that specific subsets of FRUM neurons are necessary for particular steps of courtship and copulation. In these neurons, FRUM function has been shown to be important for determining sex-specific neuronal characteristics, such as neurotransmitter profile and morphology.

Results

We identified a small cohort of FRUM interneurons in the brain and ventral nerve cord by their co-expression with the transcription factor Engrailed (En). We used an En-GAL4 driver to express a fruM RNAi construct in order to selectively deplete FRUM in these En/FRUM co-expressing neurons. In courtship and copulation tests, these males performed male courtship at wild-type levels but were frequently sterile. Sterility was a behavioral phenotype as these En-fruMRNAi males were less able to convert a copulation attempt into a stable copulation, or did not maintain copulation for long enough to transfer sperm and/or seminal fluid.

Conclusions

We have identified a population of interneurons necessary for successful copulation in Drosophila. These data confirm a model in which subsets of FRUM neurons participate in independent neuronal circuits necessary for individual steps of male behavior. In addition, we have determined that these neurons in wild-type males have homologues in females and fru mutants, with similar placement, projection patterns, and neurochemical profiles.

Keywords:
Courtship; Copulation; Drosophila; Fruitless; Engrailed; Central nervous system