Table 3

Details of antibodies used for fluorescent immunohistochemistry
Primary antibodies Species Dilution Company
B0AT3 Rabbit 1:200 Custom made (Innovagen, Sweden)
B0AT3 Mouse 1:50 Sigma-Aldrich, USA
NeuN Mouse 1:400 Millipore, Sweden
MAP2 Mouse 1:500 Sigma-Aldrich, USA
Gad67 Mouse 1:200 Millipore, Sweden
VIAAT Mouse 1:300 Synaptic Systems, Germany
Synaptophysin Mouse 1:200 BD Transduction lab, Sweden
GFAP Chicken 1:400 AbCam, United Kingdom
Pan-cytokeratin Mouse 1:200 Sigma-Aldrich, USA
PAG Mouse 1:100 AbCam, United Kingdom
VGLUT1 Guinea pig 1:500 Innovagen, Sweden, based on sequences described in [40,67].
VGLUT2 Guinea pig 1:500 Innovagen, Sweden, based on sequences described in [40,67].
Secondary antibodies Species Dilution Company
Anti-rabbit-594 Donkey 1:400 Invitrogen, USA
Anti-guinea pig-488 Goat 1:400 Invitrogen, USA
Anti-mouse-488 Goat 1:400 Invitrogen, USA
Anti-chicken-488 Goat 1:400 Invitrogen, USA

Hägglund et al.

Hägglund et al. BMC Neuroscience 2013 14:54   doi:10.1186/1471-2202-14-54

Open Data