B0AT3 immunoreactivity in vesicles, GABAergic and other neurons. Immunohistochemistry on mouse brain and spinal cord with B0AT3 immunoreactivity (red), markers (green), and nucleus marker DAPI (blue). (A) B0AT3 was extensively expressed in cortex (Bregma 0.02) together with NeuN positive cells and found in the soma and axon (B) The neuronal marker MAP2 and B0AT3 were found together in cortex (Bregma −0.70). (C) The immunoreactivity of the GABAergic marker Gad67 overlapped with the expression of the B0AT3 transporter in cortex (Bregma −0.10). (D) Highly overlapping expression was seen for B0AT3 and the presynaptic GABAergic neuronal marker VIAAT in hippocampus (Bregma −3.40). (E) Immunoreactivity of B0AT3 and the vesicular marker synaptophysin extensively overlapped in spinal cord (upper vertebrae L2 lumbar). (F) No overlap was seen with the astrocyte marker GFAP and B0AT3 in hypothalamus (Bregma −0.82). (G) No protein overlap was seen between B0AT3 and pan-cytokeratin (Bregma −2.30, around the third ventricle). Confocal pictures on paraffin sections taken with 63x magnification.
Hägglund et al. BMC Neuroscience 2013 14:54 doi:10.1186/1471-2202-14-54