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Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system

Maria GA Hägglund1, Sofie V Hellsten1, Sonchita Bagchi1, Anna Ljungdahl1, Victor CO Nilsson1, Sonja Winnergren1, Olga Stephansson1, Juris Rumaks2, Simons Svirskis2, Vija Klusa2, Helgi B Schiöth1 and Robert Fredriksson1*

Author Affiliations

1 Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden

2 Department of Pharmacology, Faculty of Medicine, University of Latvia, Riga, Latvia

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BMC Neuroscience 2013, 14:54  doi:10.1186/1471-2202-14-54

Published: 14 May 2013

Additional files

Additional file 1: Figure S1:

Characterization of the B0AT3 antibody. The antibody specificity was investigated by immunohistochemistry on mouse CNS tissues. Double immunohistochemistry with the custom made polyclonal B0AT3 antibody made in rabbit (red) and the commercial available B0AT3 antibody made in mouse (green) on brain (row A) and spinal cord (row B) tissue. The cell nucleus marker DAPI was stained in blue. (A) Co-localization of B0AT3 antibodies in cells in cerebral cortex in brain (Bregma -1.06). (B) Co-localization of B0AT3 antibodies in motor neurons and other cells in spinal cord (L2). Scale bar: 10 μm. The immunohistochemistry indicated that the custom made polyclonal B0AT3 antibody was epitope specific. Figure S2. Protein localization of B0AT3 to glutamatergic neurons and vesicles. Red staining is B0AT3, green staining is VGLUT1 and VGLUT2 respectively and blue is DAPI. (A) Overlapping expression between B0AT3 and VGLUT1 in cerebral cortex in the brain. (B) Overlapping expression for the vesicular marker VGLUT2 and B0AT3 in in cerebral cortex in the brain. Table S1. CNS expression of Slc6a17 mRNA in mouse brain. The scale of estimated Slc6a17 mRNA expression in the table; (+++) high expression, (++) medium expression, (+) low expression, and (-) not detected.

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