Table 2

Antibodies and dilutions used for all immunofluorescent stainings
Primary antibodies Secondary antibodies
Marker Type Manufacturer Dilution Type Manufacturer Dilution
CRH Goat polyclonal Santa Cruz biotechnology, Heidelberg, Germany 1:250 Donkey anti-goat Invitrogen, Breda, the Netherlands 1:100
NeuN Mouse monoclonal Chemicon, Amsterdam, the Netherlands 1:200 Donkey anti-mouse Invitrogen, Breda, the Netherlands 1:500
GFAP Mouse monoclonal Santa Cruz biotechnology, Heidelberg, Germany 1:1000 Donkey anti-mouse Invitrogen, Breda, the Netherlands 1:500
IBA-1 Goat monoclonal Santa Cruz biotechnology, Heidelberg, Germany 1:200 Donkey anti-goat Invitrogen, Breda, the Netherlands 1:400
CD-45 Rat polyclonal Serotec, Düsseldorf, Germany 1:1000 Donkey anti-rat Invitrogen, Breda, the Netherlands 1:500

The fluorophore of all secondary antibodies was Alexa Fluor® 594.

Zalachoras et al.

Zalachoras et al. BMC Neuroscience 2013 14:5   doi:10.1186/1471-2202-14-5

Open Data