Figure 5.

qPCR analysis of gene expression 3 or 7 days after a single injection. A. Relative expression of the SRC-a1/SRC-1e 3 ratio days after a single injection of AONs. AON treatment targeting exon 22 of SRC-1e leads to a 2-fold difference of the expression ratio of the two isoforms in favour of SRC-1a (independent t-test, t(6)=2.414, p<0.05, n=6-7 per group). B. Relative expression of the SRC-1a/SRC-1e ratio 7 days after a single injection of AONs. AON treatment targeting exon 22 of SRC-1e lead to 1.5-fold difference of the expression ratio of the two isoforms in favour of SRC-1a (independent t-test t(8)=2.420, p<0.05, n=5 per group). C, D. Treatment with AON targeting exon-22 of SRC-1e had no effect on total SRC-1 expression compared to control 3 (C) or 7 days (D) after a single injection (independent t-tests, t(11)=0.006, p>0.99 and t(7)=1.304, p>0.57 respectively, n=4-7 per group). E, F. GR expression remained unchanged between animals injected with AON targeting exon 22 of SRC-1e and controls 3 (E) or 7 days (F) after a single injection (independent t-tests, t(11)=0.479 p>0.64 and t(7)=0.662, p>0.52, n=4-7 per group).

Zalachoras et al. BMC Neuroscience 2013 14:5   doi:10.1186/1471-2202-14-5
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