The effects of a Ca2+ionophore and phosgene (CG) on intracellular Ca2+ changes in differentiated vs. undifferentiated M17 cells. M17 cells were cultured in Transwell inserts without and with RA differentiation to 80% confluency. They were then exposed to 5 μM Ca2+ ionophore, A23187 or 0 and 16 ppm CG. Intracellular free Ca2+ levels were monitored using Fluo-4 Ca2+ indicator assay. (A) Change in intracellular Ca2+ following A23187 exposure (N=3); (B) Change in intracellular Ca2+ following either 0 ppm (air) or 16 ppm CG exposure for 8 min (N=13). *p<0.05 when compared to treated (A23187 or CG) cells.
Andres et al. BMC Neuroscience 2013 14:49 doi:10.1186/1471-2202-14-49