Establishment of primary hippocampal cultures on PLL- and nECM-coated coverslips. Hippocampal cells were seeded either on PLL- or nECM-coated coverslips and culture progression was observed on both substrates for 7 days. (A) Bright field microscopic images taken at 24 h, 72 h and 7 days of culture, respectively. Scale bars, 100 μm. (B) Immunofluorescence analyses (×10 objective). Neuronal and glial cells grown for 24 h, 72 h and 7 days on both substrates were fixed and immunolabelled with anti-MAP2 (green) and anti-GFAP (red) antibodies, respectively. Cell nuclei were counterstained with Hoechst (blue). (C) Magnification (×20 objective) of immunofluorescence images shown in panel B. All images are representative of three independent experiments.
García-Parra et al. BMC Neuroscience 2013 14:48 doi:10.1186/1471-2202-14-48