Effect of acute nicotine treatment on FRET efficiency of α7-Cerulean and α7-Venus subunits in the presence or absence of RIC-3. Equimolar amounts of α7-Venus and α7-Cerulean were transfected either without RIC-3 or with RIC-3 at a 1:1 ratio to the nAChR subunit. Cells were incubated at various concentrations of nicotine (0, 0.1, 1 and 10 μM) at 37°C for 30 min prior to imaging. Whether RIC-3 was present or absent, there was no change in FRET efficiency between α7-Venus and α7-Cerulean subunits over increasing concentrations of nicotine (30 min) (p = 0.3, and p = 0.12, Kruskal-Wallis rank sum tests, respectively). However, the FRET efficiency between α7-Venus and α7-Cerulean was signficantly greater in the presence than the absence of RIC-3 at each of the nicotine concentrations (*, p < 0.05, **, p < 0.01, Wilcoxon rank sum test or t-test).
Dau et al. BMC Neuroscience 2013 14:47 doi:10.1186/1471-2202-14-47