MnTBAP protects against MLP. The animal experiment, spinal cord injury, and tissue processing were described in the Methods section. After the epicenter was located, the sections 0.55 to 2.55 mm rostral to the epicenter were immuno-stained at 0.5-mm intervals with an anti-HNE antibody. Upper panel, photomicrographs of anti-HNE antibody immuno-stained sections. A, lower magnification of a HNE stained spinal cord section showing the morphology of cord section and indicating the cell counting area defined by horizontal and vertical lines drawn crossing the central canal in the center of the sections, as described in cell counting in the Methods section. B and C, higher magnification of HNE stained spinal cord sections at 1.55 mm rostral to the epicenter. B, saline-treated and C, MnTBAP-treated sections. The colored panels (D-F) show double-immuno-fluorescence staining with anti-HNE (D, green) and anti-NSE (E, red) antibodies, and immuno-colocalization of D and E (F, yellow). The color photomicrographs are at a higher magnification. Scale bars are 100 μm. Lower panel, spatial profile of MnTBAP protection against MLP. The HNE-positive neurons were counted, and the counts compared between MnTBAP-treated and the vehicle-treated animals at different distances from the epicenter. MnTBAP significantly (indicated by an asterisk*) reduced the number of HNE-positive neurons in the sections 1.55 to 2.55 mm from the epicenter. Error bars, mean ± S.E.M.
Liu et al. BMC Neuroscience 2013 14:23 doi:10.1186/1471-2202-14-23