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Open Access Highly Accessed Research article

Systematic and quantitative mRNA expression analysis of TRP channel genes at the single trigeminal and dorsal root ganglion level in mouse

Ine Vandewauw, Grzegorz Owsianik and Thomas Voets*

Author Affiliations

Laboratory of Ion Channel Research, Department of Cellular and Molecular Medicine and TRPLe (TRP Research Platform Leuven), KU Leuven, Leuven, Belgium

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BMC Neuroscience 2013, 14:21  doi:10.1186/1471-2202-14-21

Published: 14 February 2013

Additional files

Additional file 1: Figure S1:

Examples of quality control measurements of total RNA extractions from different DRGs. Figure S2. Optimization of cDNA libraries for quantitative PCR analysis. Figure S3. Expression of TRP mRNAs in each individual segment of the vertebral column.

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Additional file 2: Table S1:

Relative expression levels of the different TRP channel genes in individual ganglia. Expression levels of different mice were pooled per segment and expressed as mean ± SEM. C1-7 – DRGs in cervical segments; T1-13 – DRGs in thoracic segments; L1-6 – DRGs in lumbar segments; S1 – DRG in sacral segment.

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Additional file 3:

MIQE guidelines checklist.

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Additional file 4: Table S2:

Ct values for the reference mRNA genes of individual DRGs per segment. The mean was calculated of experiments done in triplicate.

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