WT and CCR5-/- mice were infected with 104 PFU of HSV-1 by intracranial route and brain removed at 1dpi (n = 4). Brain-sequestered cells were counted and then stained with specific antibodies. Neutrophils were characterized as CD11+ Ly6G+ cells and T lymphocytes were characterized as CD3+ TCD4+ or CD3+ TCD8+. Flow cytometry, according to size and granularity, was performed as analysis. Number of leukocyte (A), neutrophils (B), T lymphocytes CD8+ (C) and CD4+ (D) were evaluated in WT and CCR5-/- mice. Results are expressed as mean ± SD and *p < 0.05, ***p < 0.001 when compared to non-infected mice. Representative gating strategy utilized for analysis of lymphocytes and granulocytes in the brain. Granulocytes population was isolated and collected for analysis. At this region, neutrophils were characterized as CD11+ Ly6G+ cells. Lymphocyte population was isolated and the cell population positive for CD3+ was collected for analysis. T lymphocytes were defined as CD3+ TCD4+ or CD3+ TCD8+. Flow cytometry data from a CCR5-/- infected mice (E). Statistical analysis used: one-way ANOVA with Tukey correction.
Vilela et al. BMC Neuroscience 2013 14:19 doi:10.1186/1471-2202-14-19