ALA responds to anterior and posterior picking-touch stimuli. (A) a schematic drawing of ALA (red), and the proprioceptor neurons PVD and FLP (grey). The locations where anterior and posterior mechanical stimuli were applied are denoted with black bars (schematic adapted from wormatlas.org). GCaMP3 fluorescence levels in ALA before and after administering anterior (B) or posterior (C) picking-touch stimuli at t=0 with a platinum wire pick. The scale bar represents a 100% deviation from the mean baseline fluorescence. Mean ± s.e.m, N=9-12 animals. (D) GFP fluorescence levels under the same conditions as in (B-C). Mean ± s.e.m, N=10 animals. Dashed lines in panels (B-D) are provided as a guide to the eye. (E) The mean GCaMP fluorescence levels during 20 sec intervals, recorded 1 minute prior to administering an anterior stimulus, immediately after the stimulus, and every 5 minutes thereafter. Mean ± s.e.m, N=10 animals. Asterisks denote a significant difference from the baseline mean fluorescence (p < 0.05).
Sanders et al. BMC Neuroscience 2013 14:156 doi:10.1186/1471-2202-14-156