Additional file 4: Figure S4.

Non-Synergistic phosphorylation of P38 and Akt upon FP and EP treatments. Time-course of quantified P38, and Akt phosphorylations at 20 and 60 minutes following (a) FGFb (50 ng/ml)-PACAP (100 ng/ml), and (b) EGF (50 ng/ml)-PACAP (100 ng/ml) treatment. Fold changes of (i) pP38, and (ii) pAkt were quantified by densitometry and normalized to the levels of actin. Significant differences between combinatorial experimental treatment of growth factor-PACAP and summation of their individual effects were calculated using the paired Student’s t-test. A value of p<0.05 was considered significant.

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Seow et al. BMC Neuroscience 2013 14:153   doi:10.1186/1471-2202-14-153