P90RSK is regulated by Erk and JNK in the NP and FP systems, but only by Erk in the EP system. (a), (b), (c) Time-course measurement of pP90RSK at 20 and 60 minutes following NGF (50 ng/ml)-PACAP (100 ng/ml), FGFb (50 ng/ml)-PACAP (100 ng/ml), and EGF (50 ng/ml)-PACAP (100 ng/ml) treatment, respectively, in the presence or absence of MEK inhibitor, U0126 (20 μM), or JNK inhibitor, SP600125 (10 μM). Significant differences between treatments with and without inhibitors were calculated using the paired Student’s t-test. A value of p < 0.05 was considered significant (**p < 0.01).
Seow et al. BMC Neuroscience 2013 14:153 doi:10.1186/1471-2202-14-153