Net inhibitor-induced reduction in neurite length is greater in the synergistic systems than in the additive effect of the single ligand treatments. Net reduction in neurite outgrowth in the (a) NP system following treatment with various concentrations of (i) U0126 (MEK inhibitor) and (ii) SP600125 (JNK inhibitor). Reductions in neurite outgrowth in the (b) FP and (c) EP systems in the presence of specific kinase inhibitors. Significant differences between the effects of the combinatorial treatment of growth factor-PACAP (bi-ligand) versus the sum of the effects for each single ligand treatment were compared using the paired Student’s t-test. A value of p < 0.05 was considered significant (**p < 0.01; *p < 0.05).
Seow et al. BMC Neuroscience 2013 14:153 doi:10.1186/1471-2202-14-153