Synergistic and sustained phosphorylation of Erk and JNK upon FP and EP treatments. Time-course of quantified Erk and JNK phosphorylation at 20 and 60 minutes upon (a) FGFb (50 ng/ml)-PACAP (100 ng/ml) (FP) or (b) EGF (50 ng/ml)-PACAP (100 ng/ml) (EP) treatment. Fold-change in (i) pErk, and (ii) pJNK were quantified by densitometry and normalized to the levels of actin. Significant differences between combinatorial experimental treatment of growth factor-PACAP and the summation of their individual effects were calculated using the paired Student’s t-test. A value of p < 0.05 was considered significant (**p < 0.01; *p < 0.05).
Seow et al. BMC Neuroscience 2013 14:153 doi:10.1186/1471-2202-14-153