Effects of 6-OHDA-lesion and STN-HFS on thalamic, pallidal and nigral VGLUT1-3 expression. A-C, Histograms show the mean ± standard error of the mean (SEM) of optical density values expressed as a percentage of values of control rats (non lesioned and non stimulated). Data were analyzed for each brain structure by Kruskal-Wallis tests with SigmaStat 3.1 software. Post-hoc analyses were carried out with the Dunn’s method. Kruskal-Wallis tests: (VGLUT1, Thalamus VL/VM, p = 0.036; STN, p = 0.536), (VGLUT2, Thalamus (VL/VM); p = 0.036; STN, p = 0.015; SNr, p = 0.049; GP, p = 0.048), (VGLUT3, Thalamus (VL/VM), p<0.001. a-l, Photographs of immunoradioautograms obtained by incubating coronal rat-brain sections with affinity-purified anti-VGLUT1 (a-d), anti-VGLUT2 (e-h) and anti-VGLUT3 (i-l) antisera and then 125I-labeled anti-rabbit IgG. GP, Globus Pallidus; SNr, Substantia nigra pars reticulata; STN, Subthalamic nucleus; Tha, Thalamus (VL/VM). *, controls vs 6-OHDA rats; #, controls vs 6-OHDA + STN-HFS rats; Δ, 6-OHDA rats vs 6-OHDA + STN-HFS rats: p < 0.05. Scale bar, 0.4 mm.
Favier et al. BMC Neuroscience 2013 14:152 doi:10.1186/1471-2202-14-152