Reduction of SCI-induced cell loss by MnTBAP + L-NA. MnTBAP (10 mg/kg) plus L-NA (1 mg/kg) was given i.p. at 15 min pre- and 6 h post-SCI (50 g.cm) and the rats were sacrificed at 12 h post-SCI. Upper panel: photomicrographs of the ventral gray matter in the CV-stained spinal cord sections at 2 mm rostral from the epicenter. A-C: lower magnification, D-F: higher magnification of A-C. Clearly, the combination of MnTBAP and L-NA treatment increased the number of surviving cells in the gray matter of the cord. Scale bars shown in C and F: 100 and 50 μm, respectively. Lower panel: spatial protection by MnTBAP + L-NA against losses of total cells in gray matter (a), glial cells in white matter (b), and motoneurons (c). * indicates a significant difference between the combination-treated and the vehicle-treated groups (p < 0.05). Error bars, mean ± SEM. Compared to vehicle-treated rats, the combination significantly reduced cell loss in both the gray and the white matter.
Ling et al. BMC Neuroscience 2013 14:146 doi:10.1186/1471-2202-14-146