U0126 alters the time constants of inactivation. A. Representative two-electrode voltage clamp IA recordings for Saline, U0126, PD98059 treatment groups. Overlaid traces are leak-subtracted currents elicited by a step to +20 mV after a -90 mV prepulse. B. Kinetics of IA inactivation were determined by fitting the +20 mV current traces with a double exponential function. The mean fast (top panel) and slow (bottom panel) time constants (τ) of inactivation were plotted ± S.D for Saline, U0126, and PD98059 treatment groups. Data from drug alone and drug with 5 nM DA samples were pooled, as both means and variance between two groups were not different (n ≥ 10 for each group). Asterisks indicate significant differences from control (saline). Both the fast and slow τ values for U0126 were significantly different than PD98059 or Saline. Saline and PD98059 were not significantly different. Data were analyzed with ANOVAs and Tukey’s post hoc tests.
Rodgers et al. BMC Neuroscience 2013 14:143 doi:10.1186/1471-2202-14-143