Unilateral CCI to the somatosensory cortex impairs sensorimotor coordination and triggers locomotor hyperactivity in adult mice. (a) CCI significantly impairs sensorimotor coordination at 1 day post-procedure as measured by the latency to fall from an accelerating rotarod (*** p < 0.001 moderate CCI versus naïve and mild CCI versus naïve). Accelerating rotarod testing at 29 days post-procedure reveals a recovery in sensorimotor coordination in moderate and mild CCI mice. The mean scores show a gradation of sensorimotor impairment with injury severity with moderate CCI mice performing worse than mild CCI. (b) CCI triggers locomotor hyperactivity in adult mice. There is no significant difference between CCI mice and naive mice at 1 day post-procedure but moderate CCI mice did travel significantly greater distances when compared to sham at this acute time point (* p < 0.05). Mild and moderate CCI mice travel significantly greater distances in open-field testing at 10 days post-procedure (*** p < 0.001 moderate CCI versus naïve, * p < 0.05 mild CCI versus naïve) and 29 days post-procedure (* p < 0.05 moderate CCI versus naïve, *** p < 0.001 mild CCI versus naïve). The performance of shams was not significantly different from naïve on either test at any post-procedure time point indicating these behavioral changes were due to the effects of cortical contusion. Data shown as mean ± SEM with n = 12/group, except open-field testing at 10 days post-procedure n = 10/group.
Radomski et al. BMC Neuroscience 2013 14:142 doi:10.1186/1471-2202-14-142