Table 2

Fluxes of the basic model
Fluxes in μM·h−1
Basic model
Reaction (group) maxApo minApo maxDeg minDeg
ROS production (DA) 19.00 19.00 19.56 19.00
ROS production (mitochondria, complex I) 21.15 0.00 0.00 0.00
ROS production (mitochondria, complex III) 46.04 26.05 35.06 23.35
ROS elimination (DJ-1) 0.00 0.00 0.00 0.00
ROS damage (protein) 14.98 22.73 35.17 20.31
ROS damage (mitochondria) 50.00 25.92 28.23 26.21
ROS damage (DNA) 5.00 0.00 0.00 5.00
Apoptosis (LBs) 35.00 0.00 0.00 0.00
Apoptosis (mitochondria) 65.00 0.00 2.40 6.47
Degradation (lysosome) 4.98 10.58 27.27 0.00
Degradation (proteasome) 0.00 0.00 0.00 0.00
Degradation (mitophagy) 0.00 10.43 17.73 0.00
Biogenesis of mitochondria 5.00 0.00 0.00 5.00
Extracellular DA 0.80 0.80 0.80 0.80
αSYN aggregation 36.00 9.89 4.24 10.00
αSYN output reaction 0.00 0.00 0.00 0.00
Toxic effect of αSYN aggregates 0.00 0.00 0.00 0.00
Inhibition of αSYN aggregation by DJ-1 1.00 9.89 4.24 10.00

The table shows the FBA results of the basic dopaminergic nerve cell experiment. Four different target functions were selected for the simulation: (i) minApo, (ii) maxApo, (iii) minDeg, (iv) maxDeg. The table includes three reactions groups: ‘ROS production (dopamine)’, ‘ αSYN aggregation’ and ‘Toxic effects of αSYN aggregates’. ‘ROS production (dopamine)’ comprise all reactions of the dopamine synthesis and metabolism, where ROS or ROS-like metabolites are produced. These reactions are DOPALlikeROS, SalsolinollikeROS, DopaminesyntheseROSout, DOPALSynthesis2, and HVAldehydSynthesis2. ‘ αSYN aggregation’ includes the reaction of DA and αSYN (SNCADopamineAggregation), the reaction of ROS and αSYN (SNCAROSAggregation), and the increased αSYN expression (SNCAOverexpression). Finally, ‘Toxic effects of αSYN aggregates’ contains the reaction MitochondriaDamageSNCAAggregates and isdamagedProtein. For each reaction group, the reaction fluxes are added up to obtain a better overview of the corresponding topic.

Büchel et al.

Büchel et al. BMC Neuroscience 2013 14:136   doi:10.1186/1471-2202-14-136

Open Data