Ubiquitin and α-synD135 associate preferentially with PS129α-syn deposits in hA30Pα-syn mice, regardless of parkin expression status. Epifluorescence microscopy illustrating the association between (A) PS129α-syn and ubiquitin, (B) PS129α-syn and α-synD135, and (C) ubiquitin and α-synD135, in hemi-brain sections from a representative symptomatic hA30Pα-syn mouse with normal parkin alleles (bregma -5.8). In blue: nuclear DNA stained with DAPI. The left micrographs illustrate representative regions immunostained for each marker, at low magnification (Scale bars: 1000 μm), and the associated triad of micrographs are higher magnifications of areas within these regions (Scale bars: 20 μm). (D) Quantitative analysis of the proportion of colabeled neuronal cell bodies and processes in hA30Pα-syn with two or no functional parkin alleles. (n = 6–10).
Fournier et al. BMC Neuroscience 2013 14:135 doi:10.1186/1471-2202-14-135