A new antibody reveals the presence of α-synD135 in the LB of PD cases. (A) Immunoblot analysis with recently developed antibodies directed against recombinant α-syn and its truncated versions. (B) Colocalization analysis of α-synD135 and PS129α-syn immunoreactivities within LB, in a melanized dopaminergic neuron (brightfield image) in the substantia nigra of a representative PD patient. (C) Control experiment showing that the staining for α-synD135 in LB is abolished when the antibody is absorbed with the truncated, but not with the full-length, recombinant α-syn protein before immunohistochemistry. The blue signal corresponds to nuclear DNA stained with DAPI. Scale bar: 10 μm.
Fournier et al. BMC Neuroscience 2013 14:135 doi:10.1186/1471-2202-14-135