Cells in CE neurospheres activate rod photoreceptor-specific regulatory gene expression. CE neurospheres were cultured in the presence of PN1CM for 20 days and rod photoreceptor regulatory gene expression  was examined (A). Q-PCR analysis revealed a temporal increase in levels of genes corresponding to the regulators of rod differentiation (Crx, Nrl, and Nr2e3) and maturation (Rhodopsin kinase, Rhodopsin, Gnat1, Phosducin, Recoverin, and Arrestin) in differentiation conditions, compared to controls (B). C = control (CE untreated cells).
Del Debbio et al. BMC Neuroscience 2013 14:130 doi:10.1186/1471-2202-14-130