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## Figure 1.
Immunohistochemical staining for parvalbumin (PV) of EE and SE rats, and cell-counting
for PV and calbindin D-28k (CalB) in the basolateral amygdala (BLA). Photomicrographs show staining for PV of EE (A, B) and SE (C, D) rat amygdala area. High density of PV-positive neurons in BLA [the lateral (LA)
and basal nuclei (BA)] was observed (A, C). B and D, higher magnification of the square
area in A and C. PV-positive neurons differed in cell sizes—large immunoreactive cells
(rectangular diameter of perikaryon > 25 μm, arrows) and small immunoreactive cells
(rectangular diameter of perikaryon < 25 μm, arrowheads). The total number of immunoreactive
cells in BLA was compared between the SE and EE rats (E and F). In the EE rats, the number of PV-positive neurons was increased (B, E), while the
number of CalB-positive neurons in the EE rats was comparable to that in the SE rats
(F). Data represented mean value per section. * Significant difference from SE rats,
p < 0.05. Scale bars = 25 μm in B and D.
Urakawa |