Immunohistochemical staining for parvalbumin (PV) of EE and SE rats, and cell-counting for PV and calbindin D-28k (CalB) in the basolateral amygdala (BLA). Photomicrographs show staining for PV of EE (A, B) and SE (C, D) rat amygdala area. High density of PV-positive neurons in BLA [the lateral (LA) and basal nuclei (BA)] was observed (A, C). B and D, higher magnification of the square area in A and C. PV-positive neurons differed in cell sizes—large immunoreactive cells (rectangular diameter of perikaryon > 25 μm, arrows) and small immunoreactive cells (rectangular diameter of perikaryon < 25 μm, arrowheads). The total number of immunoreactive cells in BLA was compared between the SE and EE rats (E and F). In the EE rats, the number of PV-positive neurons was increased (B, E), while the number of CalB-positive neurons in the EE rats was comparable to that in the SE rats (F). Data represented mean value per section. * Significant difference from SE rats, p < 0.05. Scale bars = 25 μm in B and D.
Urakawa et al. BMC Neuroscience 2013 14:13 doi:10.1186/1471-2202-14-13