Additional file 1: Figure S1.

Serial reconstruction of the imaged field. A) Synaptic boutons loaded with FM1-43 dye before (to) and after (tend) stimulation with potassium chloride; contrast phase images of the same experiment field (white box) at different magnifications: 60× (B), 40 × (C) and 20× (D). E) Serial reconstruction of 20× cell phase images of the field monitorized during the experiment (white box) and surrounding areas. F) Contrast phase image al 20× magnification showing the field where the experiment was performed after fixing and labelling with the different antibody (matching field). Note the presence of distinctive hallmarks.

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Ramírez-Franco et al. BMC Neuroscience 2013 14:127   doi:10.1186/1471-2202-14-127